Resistance to ACCase and ALS inhibitors and its target site mutation in Italian ryegrass Lolium multiflorum from Henan Province. Author(s) : Xu HongLe ;  Leng QiuLi ;  Min Hong ;  Hao Rui ;  Sun LanLan ;  Xue Fei ;  Su WangCang ;  Wu RenHai Author Affiliation : Institute of Plant Protection, Henan Academy of Agricultural Science, Henan, China. Journal article : Journal of Plant Protection 2023 Vol.50 No.1 pp.224-230 ref.29  

Abstract : To clarify the resistance level to acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor herbicides and target site resistance mechanism in Italian ryegrass Lolium multiflorum populations from some areas of Henan Province, the whole-plant bioassays were conducted to determine the resistance of HNXX01, HNZMD04, HNXX05 populations against ACCase inhibitor herbicides (fenoxaprop-P-ethyl, clodinafop-propargyl, and pinoxaden) and ALS inhibitor herbicides(mesosulfuron-methyl, flucarbazone-sodium, and pyroxsulam). The ACCase and ALS target enzyme genes were cloned from these populations, and compared with amino acid sequence to find resistance-related mutations and clarify the mechanism of target site resistance. The results of resistance level test showed that HNZMD04 and HNXX05 populations were resistant to all the six herbicides compared to susceptible population HNXX01. HNZMD04 population was resistant to fenoxaprop-P-ethyl and pyroxsulam, with a relative resistance factor of 44.65 and 40.31, respectively. The relative resistance factor of HNZMD04 to clodinafop-propargyl and flucarbazone-sodium were 11.91 and 11.93, respectively. The relative resistance factor of HNXX05 population to fenoxaprop-P-ethyl and flucarbazone-sodium were27.70 and 25.67, respectively. The results showed that the ACCase gene had a D2078G mutation in HNZMD04 and HNXX05 resistant populations, with a mutation rate of 55% and 70%, respectively. The ALS gene had P197Q and P197T mutations in HNZMD04 resistant population, with a mutation rate of30% and 5%, respectively, while the ALS gene only had P197Q mutation in HNXX05 resistant population, with a the mutation rate of 15%. These results indicated that L. multiflorum populations from Zhumadian and Xinxiang developed high resistance to ACCase and ALS inhibitor herbicides. Furthermore, ACCase mutation at amino acid D2078G and ALS mutations at amino acids P197Q and P197T contributed the herbicide resistance in the L. multiflorum populations from Henan  

Resistance to ACCase and ALS inhibitors and its target site mutation in Italian ryegrass Lolium multiflorum from Henan Province.

 

Author(s) : Xu HongLe ;  Leng QiuLi ;  Min Hong ;  Hao Rui ;  Sun LanLan ;  Xue Fei ;  Su WangCang ;  Wu RenHai

Author Affiliation : Institute of Plant Protection, Henan Academy of Agricultural Science, Henan, China.

Journal article : Journal of Plant Protection 2023 Vol.50 No.1 pp.224-230 ref.29

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Abstract : To clarify the resistance level to acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor herbicides and target site resistance mechanism in Italian ryegrass Lolium multiflorum populations from some areas of Henan Province, the whole-plant bioassays were conducted to determine the resistance of HNXX01, HNZMD04, HNXX05 populations against ACCase inhibitor herbicides (fenoxaprop-P-ethyl, clodinafop-propargyl, and pinoxaden) and ALS inhibitor herbicides(mesosulfuron-methyl, flucarbazone-sodium, and pyroxsulam). The ACCase and ALS target enzyme genes were cloned from these populations, and compared with amino acid sequence to find resistance-related mutations and clarify the mechanism of target site resistance. The results of resistance level test showed that HNZMD04 and HNXX05 populations were resistant to all the six herbicides compared to susceptible population HNXX01. HNZMD04 population was resistant to fenoxaprop-P-ethyl and pyroxsulam, with a relative resistance factor of 44.65 and 40.31, respectively. The relative resistance factor of HNZMD04 to clodinafop-propargyl and flucarbazone-sodium were 11.91 and 11.93, respectively. The relative resistance factor of HNXX05 population to fenoxaprop-P-ethyl and flucarbazone-sodium were27.70 and 25.67, respectively. The results showed that the ACCase gene had a D2078G mutation in HNZMD04 and HNXX05 resistant populations, with a mutation rate of 55% and 70%, respectively. The ALS gene had P197Q and P197T mutations in HNZMD04 resistant population, with a mutation rate of30% and 5%, respectively, while the ALS gene only had P197Q mutation in HNXX05 resistant population, with a the mutation rate of 15%. These results indicated that L. multiflorum populations from Zhumadian and Xinxiang developed high resistance to ACCase and ALS inhibitor herbicides. Furthermore, ACCase mutation at amino acid D2078G and ALS mutations at amino acids P197Q and P197T contributed the herbicide resistance in the L. multiflorum populations from Henan

Resistance to ACCase and ALS inhibitors and its target site mutation in Italian ryegrass Lolium multiflorum from Henan Province.

 

Author(s) : Xu HongLe ;  Leng QiuLi ;  Min Hong ;  Hao Rui ;  Sun LanLan ;  Xue Fei ;  Su WangCang ;  Wu RenHai

Author Affiliation : Institute of Plant Protection, Henan Academy of Agricultural Science, Henan, China.

Journal article : Journal of Plant Protection 2023 Vol.50 No.1 pp.224-230 ref.29

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Abstract : To clarify the resistance level to acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor herbicides and target site resistance mechanism in Italian ryegrass Lolium multiflorum populations from some areas of Henan Province, the whole-plant bioassays were conducted to determine the resistance of HNXX01, HNZMD04, HNXX05 populations against ACCase inhibitor herbicides (fenoxaprop-P-ethyl, clodinafop-propargyl, and pinoxaden) and ALS inhibitor herbicides(mesosulfuron-methyl, flucarbazone-sodium, and pyroxsulam). The ACCase and ALS target enzyme genes were cloned from these populations, and compared with amino acid sequence to find resistance-related mutations and clarify the mechanism of target site resistance. The results of resistance level test showed that HNZMD04 and HNXX05 populations were resistant to all the six herbicides compared to susceptible population HNXX01. HNZMD04 population was resistant to fenoxaprop-P-ethyl and pyroxsulam, with a relative resistance factor of 44.65 and 40.31, respectively. The relative resistance factor of HNZMD04 to clodinafop-propargyl and flucarbazone-sodium were 11.91 and 11.93, respectively. The relative resistance factor of HNXX05 population to fenoxaprop-P-ethyl and flucarbazone-sodium were27.70 and 25.67, respectively. The results showed that the ACCase gene had a D2078G mutation in HNZMD04 and HNXX05 resistant populations, with a mutation rate of 55% and 70%, respectively. The ALS gene had P197Q and P197T mutations in HNZMD04 resistant population, with a mutation rate of30% and 5%, respectively, while the ALS gene only had P197Q mutation in HNXX05 resistant population, with a the mutation rate of 15%. These results indicated that L. multiflorum populations from Zhumadian and Xinxiang developed high resistance to ACCase and ALS inhibitor herbicides. Furthermore, ACCase mutation at amino acid D2078G and ALS mutations at amino acids P197Q and P197T contributed the herbicide resistance in the L. multiflorum populations from Henan