<b>HRAC Group 2</b> <font size='2'> (Legacy B) </font> resistant Amaranthus hybridus (syn: quitensis) from Italy

International Survey of Herbicide-Resistant Weeds

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Friday, November 1, 2024

HRAC GROUP 2 (LEGACY B) RESISTANT SMOOTH PIGWEED
(Amaranthus hybridus (syn: quitensis))

Inhibition of Acetolactate Synthase HRAC Group 2 (Legacy B)

Italy, Friuli Venezia Giulia region

INTRODUCTION		SMOOTH PIGWEED
Smooth Pigweed (Amaranthus hybridus (syn: quitensis)) is a dicot weed in the Amaranthaceae family. In Italy this weed first evolved resistance to Group 2 (Legacy B) herbicides in 2018 and infests Soybean. Group 2 (Legacy B) herbicides are known as Inhibition of Acetolactate Synthase (Inhibition of Acetolactate Synthase ). Research has shown that these particular biotypes are resistant to imazamox, and thifensulfuron-methyl and they may be cross-resistant to other Group 2 (Legacy B) herbicides. The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

QUIK STATS (last updated Nov 03, 2023 )
Common Name	Smooth Pigweed
Species	Amaranthus hybridus (syn: quitensis)
Group	Inhibition of Acetolactate Synthase HRAC Group 2 (Legacy B)
Herbicides	imazamox, and thifensulfuron-methyl
Location	Italy, Friuli Venezia Giulia region
Year	2018
Situation(s)	Soybean
Contributors - (Alphabetically)	Silvia Panozzo, Maurizio Sattin, and Laura Scarabel
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NOTES ABOUT THIS BIOTYPE

GENERAL

Maurizio Sattin

Diversity and spread of acetolactate synthase allelic variants at position 574 endowing resistance in Amaranthus hybridus in Italy.

Author(s) : Milani, A. ; Panozzo, S. ; Pinton, S. ; Danielis, R. A. ; Sattin, M. ; Scarabel, L.

Author Affiliation : Institute for Sustainable Plant Protection (IPSP-CNR), 35020 Legnaro, Italy.

Author Email : silvia.panozzo@cnr.it

Journal article : Plants 2023 Vol.12 No.2

Abstract : Poor control of Amaranthus spp. with herbicides inhibiting acetolactate synthase (ALS) has been observed for several years in soybean fields in north-eastern Italy, but to date only a few ALS-resistant populations have been confirmed. An extensive sampling of putatively resistant Amaranthus accessions was completed in the Friuli Venezia Giulia region, across an arable land area of about 3000 km². In total, 58 accessions were tested to confirm their resistance status, recognize the Amaranthus species, identify the mutant ALS alleles endowing the resistance and determine the efficacy of 3 pre-emergence herbicides. Most accessions resulted in cross-resistance to thifensulfuron-methyl and imazamox. Genomic DNA were extracted from single seeds with a newly developed protocol; an allele-specific PCR assay revealed the presence of the 574-leucine in 20 accessions, of the 574-methionine in 22, and of both alleles in 9 accessions. The two variants showed a different spatial distribution. All resistant populations were ascribed to A. hybridus. A. hybridus resistant to ALS herbicides is well-established in this Italian region and its resistance is due to two ALS mutant alleles. Metribuzin, clomazone and metobromuron can be used as alternative herbicides to be applied in pre-emergence and they should be integrated into the management strategies to limit the spread of resistance.

ISSN : 2223-7747

DOI : 10.3390/plants12020332

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ACADEMIC ASPECTS

Confirmation Tests

Greenhouse, and Laboratory trials comparing a known susceptible Smooth Pigweed biotype with this Smooth Pigweed biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.

Genetics

Genetic studies on HRAC Group 2 resistant Smooth Pigweed have not been reported to the site. There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature

Mechanism of Resistance

Studies on the mechanism of resistance of Group 2 (Legacy B) resistant Smooth Pigweed from Italy indicate that resistance is due to an altered target site. There may be a note below or an article discussing the mechanism of resistance in the Fact Sheets and Other Literature

Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes. If you have any information pertaining to the fitness of Group 2 (Legacy B) resistant Smooth Pigweed from Italy please update the database.

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CONTRIBUTING WEED SCIENTISTS

SILVIA PANOZZO

Weed Scientist Italian National Research Council (CNR) IBAF - Weed Research Unit Viale dell'Università 16 Legnaro, 35020, Padova Italy Email Silvia Panozzo

MAURIZIO SATTIN

Senior Weed Scientist Italian National Research Council (cnr) IBAF - Weed Science Unit Viale dell'Università 16 Legnaro, 35020, Padova Italy Email Maurizio Sattin

LAURA SCARABEL

Researcher Institute of Agroenvironmental and Forest Biology National Research Council (CNR) viale dell'Università 16 Legnaro (PD), 35020, Padova Italy Email Laura Scarabel

ACKNOWLEDGEMENTS
The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Italy have been instrumental in providing you this information. Particular thanks is given to Silvia Panozzo, Maurizio Sattin, and Laura Scarabel for providing detailed information.

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Herbicide Resistant Smooth Pigweed Globally
(Amaranthus hybridus (syn: quitensis))

Country

FirstYear

Situation

Active Ingredients

Site of Action

Argentina

1996

Soybean

chlorimuron-ethyl, and imazethapyr

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Argentina

2013

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Argentina

2014

Corn (maize), and Soybean

glyphosate, and imazethapyr

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Argentina

2016

Soybean

2,4-D, dicamba, and glyphosate

Multiple Resistance: 2 Sites of Action
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)
Auxin Mimics ( HRAC Group 4 (Legacy O)

Argentina

2016

Soybean

2,4-D, and dicamba

Auxin Mimics ( HRAC Group 4 (Legacy O)

Argentina

2021

Soybean

fomesafen

Inhibition of Protoporphyrinogen Oxidase ( HRAC Group 14 (Legacy E)

Bolivia

2002

Soybean

chlorimuron-ethyl, imazamox, imazethapyr, and oxasulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Bolivia

2005

Soybean

fomesafen, lactofen, and sulfentrazone

Inhibition of Protoporphyrinogen Oxidase ( HRAC Group 14 (Legacy E)

Brazil

2018

Soybean

chlorimuron-ethyl, and glyphosate

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Canada (Ontario)

2004

Corn (maize)

bentazon, and bromoxynil

PSII inhibitors - Histidine 215 Binders ( HRAC Group 6 (Legacy C3)

France

1980

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Israel

1986

Cropland

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Italy

1978

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Italy

2018

Soybean

imazamox, and thifensulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Paraguay

2022

Corn (maize), and Soybean

chlorimuron-ethyl, and glyphosate

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

South Africa

1993

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

South Africa

2024

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Spain

1985

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Switzerland

1982

Vegetables

simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Maryland)

1972

Corn (maize), and Cropland

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Virginia)

1976

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Delaware)

1977

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (North Carolina)

1980

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Pennsylvania)

1980

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (New Jersey)

1980

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (New York)

1980

Corn (maize)

atrazine, metribuzin, and simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Wisconsin)

1985

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Kentucky)

1987

Corn (maize)

atrazine, and simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Kentucky)

1992

Soybean

chlorimuron-ethyl, flumetsulam, imazaquin, imazethapyr, nicosulfuron, primisulfuron-methyl, and thifensulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Illinois)

1993

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Virginia)

1994

Corn (maize), and Soybean

imazaquin, and imazethapyr

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Illinois)

1998

Soybean

imazethapyr, and primisulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Delaware)

2000

Vegetables

imazethapyr

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Illinois)

2000

Corn (maize)

atrazine, imazethapyr, and primisulfuron-methyl

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Ohio)

2001

Soybean

flumetsulam, and thifensulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Michigan)

2002

Soybean

chlorimuron-ethyl, imazamox, and thifensulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Uruguay

2022

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Literature about Similar Cases

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Perotti, V.E., Larran, A.S., Palmieri, V.E., Martinatto, A.K., Alvarez, C.E., Tuesca, D. and Permingeat, H.R.. 2019. A novel triple amino acid substitution in the EPSPS found in a high‐level glyphosate‐resistant Amaranthus hybridus population from Argentina. Pest Management Science 75 : 1242 - 1251.

Background

The evolution of herbicide‐resistant weeds is one of the most important concerns of global agriculture. Amaranthus hybridus L. is a competitive weed for summer crops in South America. In this article, we intend to unravel the molecular mechanisms by which an A. hybridus population from Argentina has become resistant to extraordinarily high levels of glyphosate.

Results

The glyphosate‐resistant population (A) exhibited particularly high parameters of resistance (GR50 = 20 900 g ai ha−1, Rf = 314), with all plants completing a normal life cycle even after 32X dose application. No shikimic acid accumulation was detected in the resistant plants at any of the glyphosate concentrations tested. Molecular and genetic analyses revealed a novel triple substitution (TAP‐IVS: T102I, A103V, and P106S) in the 5‐enol‐pyruvylshikimate‐3‐phosphate synthase (EPSPS) enzyme of population A and an incipient increase on the epsps relative copy number but without effects on the epspstranscription levels. The novel mechanism was prevalent, with 48% and 52% of the individuals being homozygous and heterozygous for the triple substitution, respectively. In silico conformational studies revealed that TAP‐IVS triple substitution would generate an EPSPS with a functional active site but with an increased restriction to glyphosate binding.

Conclusion

The prevalence of the TAP‐IVS triple substitution as the sole mechanism detected in the highly glyphosate resistant population suggests the evolution of a new glyphosate resistance mechanism arising in A. hybridus. This is the first report of a naturally occurring EPSPS triple substitution and the first glyphosate target‐site resistance mechanism described in A. hybridus.

García, M.J., Palma-Bautista, C., Rojano-Delgado, A.M., Bracamonte, E., Portugal, J., Alcántara-de la Cruz, R. and De Prado, R.. 2019. he Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus. International Journal of Molecular Sciences 20 : 2396 - .

The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)—a superweed collected in a GR-soybean field from Cordoba, Argentina—as well as the resistance mechanisms that govern it in comparison to a susceptible population (GSH). The GRH population was 100.6 times more resistant than the GSH population. Reduced absorption and metabolism of glyphosate, as well as gene duplication of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) or its overexpression did not contribute to this resistance. However, GSH plants translocated at least 10% more ¹⁴C-glyphosate to the rest of the plant and roots than GRH plants at 9 h after treatment. In addition, a novel triple amino acid substitution from TAP (wild type, GSH) to IVS (triple mutant, GRH) was identified in the EPSPS gene of the GRH. The nucleotide substitutions consisted of ATA¹⁰², GTC¹⁰³ and TCA¹⁰⁶ instead of ACA¹⁰², GCG¹⁰³, and CCA¹⁰⁶, respectively. The hydrogen bond distances between Gly-101 and Arg-105 positions increased from 2.89 Å (wild type) to 2.93 Å (triple-mutant) according to the EPSPS structural modeling. These results support that the high level of glyphosate resistance of the GRH A. hybridus population was mainly governed by the triple mutation TAP-IVS found of the EPSPS target site, but the impaired translocation of herbicide also contributed in this resistance..

CARVALHO, S.J.P., GONÇALVES NETTO, A., NICOLAI, M., CAVENAGHI, A.L., LÓPEZ-OVEJERO, R.F., and CHRISTOFFOLETI, P.J.. 2015. DETECTION OF GLYPHOSATE-RESISTANT PALMER AMARANTH (Amaranthus palmeri) IN AGRICULTURAL AREAS OF MATO GROSSO, BRAZIL. Planta Daninha 33 : 579 - 586.

ABSTRACT - The recent introduction of Palmer amaranth (Amaranthus palmeri) in Brazilian agricultural areas may promote several changes on weed management, especially in no-till systems and in glyphosate-resistant crops, since glyphosate-resistant biotypes of A. palmeri have been frequently selected in other countries. Therefore, this research was developed in order to evaluate the glyphosate susceptibility of a Palmer amaranth biotype recently identified in the State of Mato Grosso, Brazil. For this purpose, glyphosate susceptibility of three Amaranthus biotypes was compared: A. hybridus var. patulus, collected in the State of Rio Grande do Sul - Brazil; A. hybridus var. patulus, collected in the State of São Paulo - Brazil; and A. palmeri, collected in the State of Mato Grosso - Brazil. Dose-response curves were generated for all biotypes, considering eight rates of glyphosate and six replicates. All the experiments were repeated twice. Both A. hybridus biotypes were satisfactorily controlled by glyphosate, demanding rates equal to or lower than 541.15 g a.e. ha-1 for 80% control (LD80). The A. palmeri biotype was not controlled by glyphosate in any of the assessments and required rates greater than 4,500 g a.e. ha-1 to reach LD80, which are economically and environmentally unacceptable. Comparison of the Brazilian A. palmeri biotype to the A. hybridus biotypes, as well as, to the results available in scientific international literature, led to the conclusion that the Brazilian Palmer amaranth biotype is resistant to glyphosate..

Gaines, T. A. ; Ward, S. M. ; Bukun, B. ; Preston, C. ; Leach, J. E. ; Westra, P.. 2012. Interspecific hybridization transfers a previously unknown glyphosate resistance mechanism in Amaranthus species. Evolutionary Applications 5 : 29 - 38.

A previously unknown glyphosate resistance mechanism, amplification of the 5-enolpyruvyl shikimate-3-phosphate synthase gene, was recently reported in Amaranthus palmeri. This evolved mechanism could introgress to other weedy Amaranthus species through interspecific hybridization, representing an avenue for acquisition of a novel adaptive trait. The objective of this study was to evaluate the potential for this glyphosate resistance trait to transfer via pollen from A. palmeri to five other weedy Amaranthus species (Amaranthus hybridus, Amaranthus powellii, Amaranthus retroflexus, Amaranthus spinosus, and Amaranthus tuberculatus). Field and greenhouse crosses were conducted using glyphosate-resistant male A. palmeri as pollen donors and the other Amaranthus species as pollen recipients. Hybridization between A. palmeri and A. spinosus occurred with frequencies in the field studies ranging from <0.01% to 0.4%, and 1.4% in greenhouse crosses. A majority of the A. spinosus × A. palmeri hybrids grown to flowering were monoecious and produced viable seed. Hybridization occurred in the field study between A. palmeri and A. tuberculatus (<0.2%), and between A. palmeri and A. hybridus (<0.01%). This is the first documentation of hybridization between A. palmeri and both A. spinosus and A. hybridus..

Bhowmik, P. C.. 2010. Current status of herbicide resistant weeds around the Globe. Journal of Crop and Weed 6 : 33 - 43.

The incidence and wide spread of herbicide resistant weeds is a global problem. Over the past 65 years, repeated use of herbicides has resulted in the evolution of resistant weed species. The first resistant species to triazine was discovered in 1970 in the United States. Since then, a large number of weed species has evolved resistance to several classes of herbicide. Currently, there are 334 resistant biotypes, including 190 weed species (113 dicots and 77 monocots) in over 310, 000 fields around the world Common resistant species are Chenopodium album and Amaranthus retroflexus resistant to triazine, Phalaris minor resistant to isoproturon, P. minor and P. paradoxa resistant to diclofop, Echinochloa colona resistant to propanil, Echinochloa crusgalli resistant to butachlor, Eleusine indica resistant to trifluralin, Lolium rigidum resistant to diclofop, Lactuca serriola resistant to metsuljuron, glyphosate resistance to Eleusine indica, Conyza canadensis, Lolium rigidum, and Lolium multiflorum. Multiple weed resistance to more than one class of herbicides with different modes of action has also been documented with many species. Currently there has been increased herbicide resistance to various weed species around the globe. Most common species are Lolium rigidum, Avena fatua, Amaranthus retroflexus, Chenopodium album, Setaria viridis, Echinochloa crusgalli, Eleusine indica, Kochia scoparia, Conyza canadensis, and Amaranthus hybridus..

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